Fig. 5.

In vivo evaluation of antitumor performance. a) Schematic timeline for in vivo therapeutic process using 4T1-tumor-bearing mice. b) Body weight curves of mice during the treatment period. (n = 4). c) Time-dependent changes of primary tumor volumes (n = 4). d) Growth curve of primary tumor recorded from each mouse in all groups as noted. e) The digital photograph of primary tumor dissections from different groups on Day 14. f) Primary tumor weights of each mouse afterdifferent treatments (n = 4). g) H&E, TUNEL and antigen Ki-67 immunohistochemical staining of primary tumor tissues after the above different treatments (scale bar = 200 μm). h) Immunofluorescence staining of GPX4 and HIF-1α in primary tumor tissues after the above different treatments (scale bar = 200 μm). i) Time-dependent changes of distant tumor volumes (n = 4). j) Distant tumor weights after different treatments (n = 4). Data are represented as mean values ± SD. *, ** and *** represent statistically significant differences at P < 0.05, P < 0.01 and P < 0.001, respectively.