Figure 2.

Compensatory increase in GATA3+ and IL-33R + subsets in mice lacking RORγt expression in Tregs does not impact vaccination

(A) Frequencies indicated Treg or Treg subset from Rorc^+/+ FoxP3^cre (control) or Rorc^fl/fl FoxP3^cre (cKO) mice in MLN or siLP at steady-state.

(B) Representative gating of RORγt+ Treg population from siLP of control (gray) or cKO (blue) mice.

(C and D) (C) Frequencies of indicated Tconv subsets in MLN or (D) siLP from control (gray) or cKO (blue) mice. Phenotyping data representative of 3 independent experiments. Data represents mean ± SD.

(E) Control or cKO mice were immunized with OVA+ alum at Day 0, boosted at Day 21, and assessed at Day 28 for serum antibody titers (OVA-IgG, IgG1 and IgG2c) and cytokine secretion of IL-4 after 72-h stimulation of splenocytes.

Data are pooled from 2 independent experiments and represented as mean ± SD. Unpaired t tests were used for statistical analysis. ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.