REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-CD3e (BUV 395)	BD	Cat# 565992; RRID: AB_2739443
Anti-CD4 (BUV 496)	BD	Cat# 612952; RRID: AB_2813886
Anti-CD4 (PerCP-eFluor 710)	Thermo Fisher Scientific	Cat# 46-0042-82; RRID: AB_1834431
Anti-IL-33R (BUV 563)	BD	Cat# 749324; RRID: AB_2873698
Anti-CD8a (BUV 737)	BD	Cat# 612759; RRID: AB_2870090
Anti-CD45 (BUV 805)	BD	Cat# 748370; RRID: AB_2872789
Anti-CXCR5 (BV 421)	BD	Cat# 562889; RRID: AB_2737868
Anti-CXCR5 (BV 421)	Biolegend	Cat# 145512; RRID: AB_2562128
Anti-CD62L (BV 711)	Biolegend	Cat# 104445; RRID: AB_256421
Anti-CD44 (Alexa Fluor 700)	Biolegend	Cat# 103026; RRID: AB_493713
Anti-CD25 (APC-eFluor 780)	Thermo Fisher Scientific	Cat# 47-0251-82; RRID: AB_1272179
Anti-PD-1 (PE/Cyanine7)	Biolegend	Cat# 135216; RRID: AB_10689635
Anti-GATA3 (PE-eFluor 610)	Thermo Fisher Scientific	Cat# 61-9966-42; RRID: AB_2574686
Anti-FoxP3 (APC)	Thermo Fisher Scientific	Cat# 17-5773-82; RRID: AB_469457
Anti-FoxP3 (FITC)	Thermo Fisher Scientific	Cat# 11-5773-82; RRID: AB_465243
Anti-RORγt (PE)	Thermo Fisher Scientific	Cat# 12-6981-82; RRID: AB_10807092
Anti-RORγt (BV650)	BD	Cat# 564722; RRID: AB_2738915
Anti-CD19 (BV510)	Biolegend	Cat# 115546; RRID: AB_2562137
Anti-F4/80 (BV510)	Biolegend	Cat# 123135; RRID: AB_2562622
Anti-CD11b (BV510)	Biolegend	Cat# 101245; RRID: AB_2561390
Anti-IgG-HRP	SouthernBiotech	Cat# 1033-05; RRID: AB_2737432
Anti-IgG-HRP	BioLegend	Cat# 405306; RRID: AB_31500
Anti-IgG1-HRP	Thermo Fisher Scientific	Cat# A10551; RRID: AB_2534048
Anti-IgG2c-HRP	Thermo Fisher Scientific	Cat# PA1-29288; RRID: AB_10983148
Anti-IgG2b-HRP	Thermo Fisher Scientific	Cat# M32407; RRID: AB_2536647
Anti-CD3e	Thermo Fisher Scientific	Cat# 16-0031-82; RRID: AB_468847
Anti-CD28	Thermo Fisher Scientific	Cat# 16-0281-82; RRID: AB_468921
Chemicals, peptides, and recombinant proteins
Endofit Ovalbumin	Invivogen	vac-pova-100
Alum - Alhydrogel adjuvant 2%	Invivogen	vac-alu-250
Addavax	Invivogen	vac-adx-10
Critical commercial assays
Th1/Th2 mouse uncoated ELISA kits	Invitrogen	88-7711-44
Experimental models: Organisms/strains
Mouse: C57BL/6	Jackson Laboratory	000664
Mouse: C57BL/6 FoxP3cre	Jackson Laboratory	016959
Mouse: C57BL/6 Rorctm3Litt	Jackson Laboratory	008771
Software and algorithms
GraphPad Prism 9	GraphPad	Version 9
FlowJo version 10.6.0	FlowJo	10.6.0
Other
AccuraCeck count beads	Life Technologies	PCB100
ACK lysis buffer	Invitrogen	501129751
Live/Dead viability dye	Thermo Scientific	L34962
Live/Dead viability dye	Thermo Scientific	L34966
TMB	Invitrogen	00-4201-56
FoxP3/transcription factor fixation/permeabilization reagent	Fisher Scientific	00-5523-00