Extended Data Fig. 5. Acyl-GIP effects on cFos neuronal activation in the hypothalamus in HFD-fed Vgat-Gipr KO mice.

Representative image of hypothalamic acyl-GIP^Cy5 fluorescence (n = 3-5 each group) (a) and cFos neuronal activity in the arcuate nucleus (ARC), paraventricular nucleus (PVN), ventromedial hypothalamus (VMH) and dorsomedial hypothalamus (DMH) (n = 4-5 each group) (b) in 26-wk old male C57BL/6J wild-type (WT) and Vgat-Gipr (KO) mice treated subcutaneous (s.c.) with a single dose of either vehicle or acyl-GIP^Cy5 (150 nmol/kg; n = 3-5 each group). Corresponding cFos quantification in the ARC (n = 4-5 each group) (c), PVN (n = 4-5 each group) (d), VMH (n = 4-5 each group) (e), and DMH (n = 4-5 each group) (f). CFos neuronal activity (G) and corresponding cFos quantification (H) in the lateral parabrachial nucleus (LPBN) in 26-wk old male C57BL/6J WT and Vgat-Gipr (KO) mice treated s.c. with a single dose of either vehicle or acyl-GIP^Cy5 (150 nmol/kg; n = 4-5 each group). Data in panel C-F,H were analyzed using student’s 2-sided, 2-sided, 2-tailed t-test. Scale bars in panels A and B are 100 μm, scale bars in panel G are 200 μm. Data represent means ± SEM; asterisks indicate * p < 0.05. Individual p values are shown in the Data Source file.

Source data