Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Nov 2;28(9):3943–3954. doi: 10.1038/s41380-023-02296-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — A Discrimination index for novel location recognition on day 2 and (B, C) contextual fear-conditioning freezing levels in TIMP2^fl/fl and Syn^Cre/+; TIMP2^fl/fl mice (2–3 months of age, N = 13–15 mice per group). D Proportion of mice of each genotype using non-hippocampus-dependent (gray) and hippocampus-dependent (yellow) search strategies during the testing trials of TIMP2^fl/fl and Syn^Cre/+; TIMP2^fl/fl mice in Barnes maze (2–3 months of age, N = 12–14 mice per group) with (E) quantification of the percentage of mice using these strategies on day 3. F Cognitive complexity scores for TIMP2^fl/fl and Syn^Cre/+; TIMP2^fl/fl mice based on strategies used on day 3 of Barnes maze. G Representative confocal microscopy images of BrdU⁺ and NeuN⁺ cells in DG of TIMP2^fl/fl and Syn^Cre/+; TIMP2^fl/fl mice (2–3 months of age, N = 7–8 mice per group, scale bar, 100 μm) with corresponding (H) quantification of BrdU⁺ NeuN⁺ newborn neurons in the DG. I Representative confocal microscopy images of aggrecan and homer1 puncta in molecular layer of DG of TIMP2^fl/fl and Syn^Cre/+; TIMP2^fl/fl mice (N = 7–8 mice per group, scale bar, 5 μm) with corresponding (J) quantification of aggrecan puncta and (K) co-localized puncta of aggrecan and homer1, as indicated by overlaid circles. L Schematic depicting ChABC injection paradigm with representative WFA staining following ChABC digestion in DG; scale bar, 50 μm. M Representative confocal microscopy images of DCX⁺ cells in DG of TIMP2^fl/fl and Syn^Cre/+; TIMP2^fl/fl mice injected with ChABC or vehicle (2–3 months of age, N = 6 mice per group; scale bar, 20 μm) with corresponding quantification of (N) number of DCX⁺ immature neuroblasts and (O) proportion of DCX⁺ cells in SGZ or GCL in DG of TIMP2^fl/fl and Syn^Cre/+; TIMP2^fl/fl mice. Data are represented as mean ± SEM. Student’s t test for two-group comparisons in (A–C), (H), and (J, K), chi-square test in (E), nested t-test (F) with trial and mice as levels, and 2-way RM-ANOVA, Sidak’s post-hoc test in (N, O). *P < 0.05, ** P < 0.01, ***P < 0.001, ****P < 0.0001. Data points represent individual mice.