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. 2023 Dec 8;5(12):2148–2168. doi: 10.1038/s42255-023-00936-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — a. Gene expression levels of selected SLC-encoding genes in human colorectal tissues. Values are log2(TPM + 0.001) from n = 359 normal tissue, n = 380 primary tumor and n = 1 metastatic samples. b. Gene expression levels of selected SLC-encoding genes in human breast tissues. Values are log2(TPM + 0.001) from n = 292 normal tissue, n = 1092 primary tumor and n = 7 metastatic samples. c. Heat map showing gene silencing efficiency of 4 deconvoluted siRNAs against each SLC-encoding gene. Values are mRNA expression normalized to ACTIN levels and relative to NTC control. Red boxes indicate selected siRNA for further experiments. d. Immunoblots of SLC6A14 and Vinculin (loading control) in control (NTC) or SLC6A14-silenced HCT116 cells. Blots are representative of three independent experiments. e. Immunoblots of SLC25A15 and Vinculin (loading control) in control (NTC) or SLC25A15-silenced HCT116 cells. Blots are representative of three independent experiments. f. Immunoblots of SLC12A4 and Vinculin (loading control) in control (NTC) or SLC12A4-silenced HCT116 cells. Blots are representative of three independent experiments. g. mRNA expression levels of indicated SLC-encoding genes in HCT116 p21^-/- cells versus HCT116 WT control. Values are mean±s.e.m. from n = 3 independent experiments. Data in a,b:: Box and whiskers plots are log2 (TPM + 0.001) with box limits representing 25^th and 75^th percentiles of the dataset. Whiskers extend from the hinge to the largest or smallest value, corresponding to the upper or lower 1.5x IQR (inter-quartile range). Individual dot points represent data beyond the end of the whiskers.