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. 2023 Oct 12;17(12):1223–1232. doi: 10.1111/crj.13707

FIGURE 4.

FIGURE 4

Effect of sh‐DNAJB1 and miR‐23a‐3p inhibitors on CSE‐induced PMVECs apoptosis. PMVECs were transfected with sh‐NC, sh‐DNAJB1, NC inhibitors+sh‐DNAJB1 or miR‐23a‐3p inhibitors+sh‐DNAJB1. (A) miR‐23a‐3p expression was tested using qRT‐PCR. (B–C) qRT‐PCR and WB analysis were employed to measure DNAJB1 mRNA and protein expression. (D–F) Transfected PMVECs were exposed to CSE. MTT assay (D) and flow cytometry (E) were performed to examine cell viability and apoptosis. (F) WB analysis was used to test protein levels. *, P < 0.05; **, P < 0.01; ***, P < 0.001.