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. 2023 Dec 19;13:22648. doi: 10.1038/s41598-023-49646-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparative molecular profiling of the parental and trastuzumab-resistant (TR) cell lines. (a) Immunoblot analysis of receptor tyrosine kinases (RTKs) and related downstream molecules. α-Tubulin was used as the loading control. The blots were cut prior to hybridization with antibodies. The uncropped version of the western blots is reported in Supplementary Fig. S2. (b) Cell surface expression of HER2 was determined by flow cytometry (mean fluorescence intensity, MFI). Error bars, SD.