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. 2023 Dec 18;13:22598. doi: 10.1038/s41598-023-48798-8

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© The Author(s) 2023, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Reduction of CCA cell adhesion and migration in LAMA3-silenced cells. (a) Cell adherent area analyzed by Image J (right). Data present as mean ± SEM in percent relative to those of the siNeg from three independent experiments. Representative crystal violet staining of adherent cells under 10 × magnification and the inserts represent enlarge areas (left). Scale bar: 40 µm. ECM: mock-decellularized ECM-coated plate. (b) Migration speed of CCA cells collected by cell tracking in time-lapse every 15 min for 12 h. Data from one representative experiment were shown in dot plots, in which individual dots represent the average migration speed (µm/hour) per area (n = 150). (c) Cell migration examined by the transwell assay. Graphs were created using Graphpad Prism. *P < 0.05, **P < 0.01, ***P < 0.001.