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. 2023 Dec 18;13:22598. doi: 10.1038/s41598-023-48798-8

Figure 7.

Figure 7

Representative western blotting illustrated the decrease in the pFAK/tFAK ratio and the inhibition of EMT markers expression in KKU-213 cells transfected with LAMA3 siRNA. Treated cells were reseeded for 24 h before protein extraction and GAPDH was used as the internal control. The cropped blots are used in the figure. The membranes were cut prior to exposure so that only the portion of gel containing the desired bands would be visualized. Full-length blots are presented in Supplementary Fig. S4. (a) Expression of FAK and its phosphorylation at Y397. (b) Expression of EMT-markers, including vimentin and Slug, and the epithelial markers, E-cadherin and claudin-1. Band intensity was showed as mean ± SEM in percent relative to those of the siNeg from at least four independent experiments. Graphs were created using Graphpad Prism. *P < 0.05, **P < 0.01, ***P < 0.001.