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. 2023 Dec 18;13:22598. doi: 10.1038/s41598-023-48798-8

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© The Author(s) 2023, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Alteration of E-cadherin and F-actin distribution and shrinkage of cell spreading area in CCA cells upon LAMA3 knockdown. Forty-eight-hr LAMA3-silenced cells were reseeded for 24 h before immunofluorescent staining. (a,b) Immunofluorescent images showed localization and distribution of E-cadherin protein (green), F-actin (using phalloidin staining, red), and nuclei (Hoechst staining, blue). (a) HuCCA-1; (b) KKU-213; scale bar: 40 µm. (c) The cell size in the unit area (μm²/cell) and (d) cell circularity was measured from phalloidin staining. Data from one representative experiment were shown in box plots. The value close to one indicates high circularity. (cell spreading: n > 40, circularity = n > 15). Images were captured from FV1000. **P < 0.01, ***P < 0.001.

Alteration of E-cadherin and F-actin distribution and shrinkage of cell spreading area in CCA cells upon LAMA3 knockdown. Forty-eight-hr LAMA3-silenced cells were reseeded for 24 h before immunofluorescent staining. (a,b) Immunofluorescent images showed localization and distribution of E-cadherin protein (green), F-actin (using phalloidin staining, red), and nuclei (Hoechst staining, blue). (a) HuCCA-1; (b) KKU-213; scale bar: 40 µm. (c) The cell size in the unit area (μm²/cell) and (d) cell circularity was measured from phalloidin staining. Data from one representative experiment were shown in box plots. The value close to one indicates high circularity. (cell spreading: n > 40, circularity = n > 15). Images were captured from FV1000. **P < 0.01, ***P < 0.001.