TABLE 1.
B. subtilis strains used in this study
| Strain | Relevant characteristic(s) | Description |
|---|---|---|
| BG1 | thr-5 trpC2 | Wild type |
| BG119 | BG1 pnpA::Kmr | Replacement of pnpA coding sequence with Kmr gene |
| BG149 | BD99 amyE::tetA(L)-lacZ | Wild-type tetA(L)-lacZ gene fusion integrated at amyE locus |
| BG161 | BG119 Spr | Insertion of Spr gene upstream of tetA(L) |
| BG162 | BG161 Tcr | Mutant selected for Tcr; contains A→G change in tetA(L) promoter |
| BG178 | BG161 Tcr | Mutant selected for Tcr; contains amplified tetA(L) |
| BG179 | BG161 Tcr | Mutant selected for Tcr; contains amplified tetA(L) |
| BG182 | BG161 Tcr | Mutant selected for Tcr; mutation unlinked to tetA(L) |
| BG194 | BD99 amyE::tetA(L)-lacZ | tetA(L)-lacZ fusion with A→G change in tetA(L) promoter |
| BG226 | BG1 Tcr | A→G change in promoter of tetA(L) gene in wild-type strain |