Fig. 3.
Evaluation of cytotoxicity on LLC-PK1 cells and antiviral efficacy of Tubercidin against PEDV. (A) Cytotoxicity of Tubercidin in LLC-PK1 cells. The cytotoxicity was determined by CCK-8 assay. The LLC-PK1 cells were treated or not treated with different concentrations of Tubercidin for 16 h. (B and C) Microscopic images of LLC-PK1 cells infected with DR13-GFP and GDU-GFP (0.1 MOI) treated with different concentrations of Tubercidin (0.25–1 μM). At the same time, viral fluids were collected and virus titer was determined by TCID50 assay. The experiment was performed three times independently, images were representative of results obtained from three independent experiments, scale bars = 150 μm. Differences were considered significant at P < 0.05 (*), P < 0.01 (**), P < 0.001 (***) and P < 0.0001 (****).