Fig. 8.

Effect of Tubercidin on virus replication at various intervals pre- and post-PEDV infection. (A) Add Tuberculin to Vero cells pre- and post-infection with PEDV at the specific time points. Light brown bars represent PEDV infection, light blue bars represent Tubercidin treatment. (B and C) Vero cells were infected with DR13-GFP and HB variant and treated with Tubercidin (1 μM) or 0.1 % DMSO at 2 h before infection, as well as at 2, 4, 6, 8, 10 and 12 h post-infection. After 16 h, the samples were collected for determining viral RNA copies by RT-qPCR assay. All experiments were performed with three independent replicates. Differences were considered significant at P < 0.05 (*), P < 0.01 (**), P < 0.001 (***) and P < 0.0001 (****).