Fig. 3.

Analysis of RHΔKU80 tachyzoites cell cycle after treatment. (A) hTERT monolayers infected with RHΔKU80 tachyzoites were incubated with 45 or 90 μM topotecan, 12 or 24 μM HCPT, 4 mM HU and DMSO 0.1% for 6 h and propidium iodide was used to stain DNA. Tachyzoites were analyzed by FACS. One representative experiment for each drug at the lowest concentration plus controls is shown. (B) Bar graphs showing the percentage of parasites in each phase of the cell cycle. Statistical analysis was performed with one-way ANOVA and Dunnett's multiple comparison test (****p < 0.001, **p < 0.005).