Table 1.
Reagents utilized for NK cell surface flow cytometric analyses.
| Marker | Clone | Fluorophore | Purpose | Catalog |
|---|---|---|---|---|
| CD3 | UCTH1 | FITC | Lineage exclusion (T cells) | BioLegend #300406 |
| CD14 | HCD14 | FITC | Lineage exclusion (macrophages, monocytes, granulocytes) | BioLegend #325604 |
| CD19 | HIB19 | FITC | Lineage exclusion (B cells) | BioLegend #302206 |
| CD56 | HCD56 | APC | Consensus NK cell marker | BioLegend #318310 |
| CD16 | 3G8 | Pacific Blue | NK cell marker, ADCC mediator | BioLegend #302032 |
| – | – | 7-AAD | Live/dead discriminator | Stem Cell Tech# 75001 |