TABLE 2.
Effect of plasmids with V. alginolyticus genes on growth of K+-uptake-defective E. coli strains at low K+ concentrations
| Plasmid | V. alginolyticus gene(s) present | Minimal K+ concn (mM) required for growth of straina:
|
|||||
|---|---|---|---|---|---|---|---|
| LB2003 ΔtrkA | LB670 ΔsapABCDF (“ΔtrkE”) | LB700 ΔtrkA ΔsapABCDF | LB650 ΔtrkH ΔtrkG | TK2693 ΔtrkG1 ΔtrkH92 | LB680 ΔtrkH ΔsapABCDF | ||
| pKT8 | rhlE ktrA ktrB | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 |
| pKT81 | rhlE | 30 | 3 | 30 | 30 | 30 | 3 |
| pKT82 | ktrB | 30 | 3 | 30 | 30 | 30 | 3 |
| pKT83 | rhlE ktrA | 30 | 3 | 30 | 30 | 30 | 3 |
| pKT84 | ktrA ktrB | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 |
| pKT85 | rhlE ktrA | 30 | 3 | 30 | 30 | 30 | 3 |
| pKT86 | rhlE ktrB | 10 | 1 | ND | 10 | ND | ND |
| pHG165 or no plasmid | 30 | 3 | 30 | 30 | 30 | 3 | |
a
Genotype with respect to trk genes. All strains are ΔkdpFABC5 kup-1. ND, not determined.