Table 1.
Summary of steady state kinetics for WT and R252A mutant using UDP-Glc and 1-naphthol
| Kinetic parameters | UDP-Glca |
1-naphthola |
||
|---|---|---|---|---|
| WT | R252A | WT | R252A | |
| kcat (s−1) | 0.088 ± 0.023 | 0.028 ± 0.002 | 0.20 ± 0.02 | 0.040 ± 0.003 |
| KM (μM) | 47 ± 13 | 94 ± 18 | 132 ± 16 | 100 ± 20 |
| Vmax (μM s−1) | (3.3 ± 0.3) × 10−3 | (1.1 ± 0.1) × 10−3 | (7.7 ± 0.5) × 10−3 | (1.5 ± 0.2) × 10−3 |
| kcat/KM (μM−1 s−1) | (1.9 ± 0.7) × 10−3 | (2.9 ± 0.6) × 10−4 | (1.5 ± 0.2) × 10−3 | (4.0 ± 0.9) × 10−4 |
a
Indicates the substrate whose concentration was varied.