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Primers used for real-time quantitative PCR.
| Target | Primer | Primer sequence (5′-3′) | Amplicon size |
|---|---|---|---|
| ZP3-Both | Forward | TGTGAGCCTCTGGTCTCCAT | 102 |
| Reverse | CACCAGGGCATCGTCAGTTA | ||
| ZP3-Oocyte | Forward | TACTGAGCTGTGCTACCCCC | 286 |
| Reverse | CACCAGGGCATCGTCAGTTA | ||
| ZP3-Cancer | Forward | CTGTGCAGGATCTCCACTCG | 107 |
| Reverse | CATGACCATCAGAGTGGCCT | ||
| HPRT1 | Forward | ACACTGGCAAAACAATGCAGA | 99 |
| Reverse | TTCGTGGGGTCCTTTTCACC |
