TABLE 1.
Plasmids used in this study
| Plasmid | Relevant characteristicsa | Source (reference) |
|---|---|---|
| pBluescript II SK(+) | Phagemid derived from pUC19, Plac, lacZ, Apr, f1(+) origin, ColE1 origin | Stratagene (1) |
| pET11a* | Derivative of pET11a with additional cloning sites (KpnI, XhoI, SalI), PT7lac | J. Altenbuchner, Stuttgart, Germany (42) |
| pMARRE0 | Apr; 0.982-kb PCR product of primers NH2-3 and HOM in the EcoRV site of pBluescript II SK(+) | This study |
| pMARRE1 | Apr; pBluescript II SK(+) containing a 5,657-bp KpnI fragment of R. opacus 1CP DNA | This study |
| pMARRE2 | Apr; pBluescript II SK(+) containing a ca. 7-kb XhoI fragment of R. opacus 1CP DNA | This study |
| pMARRE3 | Apr; pBluescript II SK(+) containing a 4,628-bp PstI fragment of R. opacus 1CP DNA | This study |
| pMARRE5 | Apr; 2,079 bp from pMARRE1 on EcoRI-HindIII fragment in pBluescript II SK(+) | This study |
| pMARRE10 | Apr; PCR-amplified macA in NdeI and BamHI site of pET11a* | This study |
a
Abbreviation: Apr, ampicillin resistance.