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. 2023 Nov 30;14:1301374. doi: 10.3389/fmicb.2023.1301374

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Copyright © 2023 Bacqué, Delgado, Gil, Ibarra, Benito, García-Arata, Moreno-Lorenzo, Sáez de Adana, Gómez-González, Sánchez, Montero and Thomson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bootscan analyses of 4 NFLG sequences of viruses from the BF13 cluster, 2 from Spain, obtained by us, and 2 from Italy, obtained by Saladini et al. (2012). The horizontal axis represents the position in the HXB2 genome of the midpoint of a 250 nt window moving in 20 nt increments and the vertical axis represents bootstrap values supporting clustering with subtype reference sequences. As outgroup, a reconstructed B-F1 ancestor sequence was used. Breakpoint positions of segments of potential recombinant origin detected in the bootscan analyses in at least 3 viruses are indicated on top, numbered as in the HXB2 genome. These breakpoints were located in the midpoint positions of the transitions between the 75% consensus sequences of subtype B and of the Brazilian F1 subtype variant.