FIG. 4.

The tcbC gene of pMU10 induces TdcE synthesis. (A) The subclones derived from pMU10 are shown. The ability and inability of the constructs to induce TdcE enzyme synthesis and to complement an E. coli pfl mutant are indicated by plus and minus signs, respectively. The white box at the end of the tcbC gene in pMU1013 indicates that the gene sequence is out of frame. (B) Western blot showing the ability of the various pMU10 plasmid derivatives to induce anaerobic synthesis of TdcE. Lane 1, MC4100 (10 μg of protein); lane 2, RM221 (Δpfl Δact)/pMU10; lane 3, RM221/pMU1011; lane 4, RM221/pMU1012; lane 5, RM221/pMU1013; lane 6, RM221/pBR322.