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Analysis of TcbC synthesis with the T7 polymerase/promoter system. Polypeptides were specifically labelled with [35S]methionine as described in Materials and Methods and were separated in a 12.5% (wt/vol) polyacrylamide gel containing SDS. Lane 1, K38/pGP1-2 containing pT7-4; lane 2, K38/pGP1-2 containing pT7-5; lane 3, K38/pGP1-2 containing pT7-6; lane 4, K38/pGP1-2 containing pT7-1011. The molecular masses of the standard proteins used are given.
