TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or phenotype | Reference or source |
|---|---|---|
| Strains | ||
| MC4100 | F−araD139 Δ(argF-lac)U169 ptsF25 deoC1 relA1 flbB530 rpsL150 λ− | 5 |
| FM420 | Like MC4100 but recA56 | 43 |
| RM202 | Like MC4100 but Δpfl-25 Ω(pfl::cat pACYC184) Δ(srl-recA)306::Tn10 | 32 |
| RM221 | Like MC4100 but Δ(pfl-act) Δ(srl-recA)306::Tn10 | 11 |
| RM226 | Like MC4100 but Δpfl-25 Ω(pfl::cat pACYC184) ΔtdcE | 11 |
| 234M11 | Like MC4100 but Δact Ω(act::cat pACYC184) Δ(srl-recA)306::Tn10 | 31 |
| JM109 | Δ(lac-proAB) recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 | 42 |
| W3110 | F− IN(rrnD-rrnE)1 rph-1 λ− | 18 |
| W3110pfl | Like W3110 but Δpfl-25 Ω(pfl::cat pACYC184) | This work |
| K38 | phoA tonA ampF | 22 |
| C. butyricum DSM-552 | Type strain | German Culture Collection, Braunschweig, Germany |
| Plasmids | ||
| pBR322 | Tetr Ampr | 4 |
| pT7-4 | Ampr, T7Φ10 | 34 |
| pT7-5 | Ampr, T7Φ10 | 34 |
| pT7-6 | Ampr, T7Φ10 | 34 |
| pGP1-2 | Kmr, T7 gene 1 (RNA polymerase) | 34 |
| pMU10 | Like pBR322, tcbA′ tcbB+ tcbC+ | This work |
| pMU1011 | Like pBR322, tcbC+ | This work |
| pMU1012 | Like pMU1011 but mutated in tcbC | This work |
| pMU1013 | Like pMU1011 but mutated in tcbC | This work |
| pT7-1011 | Like pT7-5 but tcbC+ | This work |
| pT7-1014 | Like pT7-1011 but with insert in the opposite orientation relative to the T7Φ10 promoter | This work |