. Author manuscript; available in PMC: 2023 Dec 20.

Published in final edited form as: Neurobiol Dis. 2023 Sep 28;187:106313. doi: 10.1016/j.nbd.2023.106313

Table 1.

Overview of lipid subclass changes at 6, 9, and 12 months

		6-month-old mice			9-month-old mice			12-month-old mice
Category	Subclass	Species detected	ANOVA p-val	Adj p-val (q), N =36	Species detected	ANOVA p-val	Adj p-val (q), N =24	Species detected	ANOVA p-val	Adj p-val (q), N =29
Glycerophospholipids	PC	134	0.7415	1	107	0.2928	0.3267	160	0.4243	0.4627
	PE	152	0.1975	1	154	0.0284^*	0.0563	119	0.5653	0.5372
	PS	62	0.1171	1	58	0.0014	0.0125	25	0.46	0.4627
	PI	19	0.0189	0.7144	17	0.0054	0.0168	34	0.0086^*	0.0338^*
	MePC	17	0.9859	1	7	0.4901	0.486	15	0.7552	0.6329
	PA	13	0.9596	1	6	0.7008	0.5957	9	0.0025^*	0.0184^*
	BisMePA	4	0.1589	1	1	0.0041	0.0168	6	0.2812	0.3647
	dMePE	9	0.195	1	2	0.2041	0.269	0	N/A	N/A
	PG	19	0.6255	1	4	0.4685	0.486	4	0.859	0.6765
	BisMePE	1	0.8072	1	0	N/A	N/A	0	N/A	N/A
	BisMePS	0	N/A	N/A	0	N/A	N/A	2	0.0001^*	0.0022^*
	CL	22	0.5812	1	0	N/A	N/A	0	N/A	N/A
	PEt	7	0.3261	1	2	0.9792	0.7283	0	N/A	N/A
	PMe	2	0.8695	1	0	N/A	N/A	0	N/A	N/A
	PIP2	2	0.1393	1	0	N/A	N/A	3	0.0161^*	0.0394^*
	PIP	1	0.7034	1	0	N/A	N/A	0	N/A	N/A
	LPC	30	0.7943	1	10	0.6835	0.5957	7	0.2805	0.3647
	LPE	23	0.9947	1	16	0.6396	0.5957	4	0.0127^*	0.035^*
	LPS	7	0.9061	1	5	0.211	0.269	2	0.0817	0.1287
	LPI	7	0.7746	1	1	0.1715	0.2551	0	N/A	N/A
	LPG	4	0.8888	1	0	N/A	N/A	0	N/A	N/A
	LdMePE	1	0.4552	1	0	N/A	N/A	0	N/A	N/A
	BiotinylPE	0	N/A	N/A	1	0.8078	0.6554	1	0.0225^*	0.0451^*
Glycerolipids	TG	107	0.5998	1	44	0.0057^*	0.0168^*	72	0.0002^*	0.0022^*
	MGDG	3	0.8693	1	2	0.0005	0.0089	5	0.0214^*	0.0451^*
	MGMG	6	0.7537	1	0	N/A	N/A	6	0.0497^*	0.0887
	DG	13	0.1381	1	70	0.1174	0.1905	59	0.6614	0.5834
	SQMG	2	0.5986	1	0	N/A	N/A	0	N/A	N/A
	SQDG	1	0.9316	1	0	N/A	N/A	0	N/A	N/A
Sphingolipids	Hex1Cer	0	N/A	N/A	85	0.0243^*	0.0542	104	0.4617	0.4627
	CerG1	50	0.5466	1	0	N/A	N/A	0	N/A	N/A
	SM	47	0.6398	1	23	0.0066	0.0168	36	0.3243	0.3973
	Cer	14	0.9804	1	6	0.0048	0.0168	21	0.4374	0.4627
	CerP	0	N/A	N/A	0	N/A	N/A	17	0.0035^*	0.0193^*
	ST	10	0.3977	1	9	0.2283	0.2717	14	0.2176	0.3199
	So	3	0.4465	1	0	N/A	N/A	0	N/A	N/A
	CerPE	0	N/A	N/A	1	0.0766	0.1367	0	N/A	N/A
	phSM	4	0.3619	1	0	N/A	N/A	0	N/A	N/A
	CerG2GNAc1	1	0.5784	1	0	N/A	N/A	0	N/A	N/A
	SPH	0	N/A	N/A	0	N/A	N/A	1	0.775	0.6329
	Hex2Cer	0	N/A	N/A	0	N/A	N/A	1	0.0112	0.035
Sterol Lipids	ChE	1	0.5341	1	1	0.918	0.7124	2	0.9151	0.6958
Sterol Lipids	ZyE	0	N/A	N/A	0	N/A	N/A	3	0.0092^*	0.0338^*
Fatty Acyls	FA	2	0.5893	1	0	N/A	N/A	0	N/A	N/A
Fatty Acyls	AcCa	0	N/A	N/A	0	N/A	N/A	2	0.5847	0.5372
Prenol Lipids	Co	0	N/A	N/A	0	N/A	N/A	1	0.0523	0.0887
Prenol Lipids	Total	800			632			735

Bold text indicates lipid subclasses significantly different between WT and LacQ140 mice (p<0.05 or q<0.05).

^(*)

Asterisk indicates lipid subclasses significantly different among other groups (p<0.05 or q<0.05).

One way ANOVA was conducted followed by Tukey’s multiple comparisons test, two-stage linear step-up procedure of Benjamini, Krieger, and Yekutieli, q<0.05.