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. 2023 Dec 19;22:262. doi: 10.1186/s12934-023-02266-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Genetic cluster architecture for the synthesis of bottromycin (A) and pamamycin (B) in recombinant S. lividans ΔYA8. The heterologous host S. lividans ΔYA8-DG2 contains a genomic copy of the 18 kb bottromycin biosynthesis-related cluster from Streptomyces sp. BC16019 [20]. The cluster is expressed under the control of synthetic P₄₁ promoters, whereby hygR displays a resistance marker introduced during the cloning process. The heterologous host S. lividans ΔYA8-R2 contains a genomic copy of the 25 kb pamamycin biosynthesis-related cluster from Streptomyces alboniger DSMZ 40043 [38]. The cluster is expressed under the control of native promoters