Table 6.

Strains and plasmids

Strains and plasmids	Description	References
Strains
E. coli DH5α	Strain for plasmid amplification	Invitrogen
E. coli ET12567	Strain harbouring pUZ8002 for conjugal gene transfer	[23]
E. coli BL21 (DE3)	Strain for high-level expression of recombinant proteins	Thermo Fisher Scientific
E. coli p21pcaI	Derivative of E. coli BL21 (DE3) harbouring p21pcaI gene	This work
E. coli p21pcaJ	Derivative of E. coli BL21 (DE3) harbouring p21pcaJ gene	This work
S. lividans ΔYA8	Derivative of S. lividans TK24, 8 secondary metabolite gene clusters were removed from genomic DNA	[18]
S. lividans ΔYA8-DG2	Derivative of S. lividans ΔYA8 containing bottromycin biosynthetic cluster	This work
S. lividans ΔYA8-R2	Derivative of S. lividans ΔYA8 containing pamamycin biosynthetic cluster	This work
Plasmids
pET21a	Vector for protein overexpression	Invitrogen
p21pcaI	Derivative of pET21a plasmid. Protein expression vector for pcaI gene of P. putida KT2440	This work
p21pcaJ	Derivative of pET21a plasmid. Protein expression vector for pcaJ gene of P. putida KT2440	This work
DG2-Km-P41hyg	Derivative of integrative DG2-cosmid with KmR marker and P41 promoter pairs, contains bottromycin biosynthetic cluster	[20]
R2	An Integrative cosmid containing pamamycin biosynthetic gene cluster	[38]