A. Study design. Tail immersion assay (N=8), locomotor activity (N=7) and pulse oximeter measurements (N=6) were performed in the same mice at different time points. B-H. Effects of increased doses of NFEPP (0.8 mg/kg s.c.) on withdrawal responses to noxious heat in a tail immersion assay (B), locomotor activity (C-F), blood oxygen saturation (G) and heart rate (H) in healthy control (HC) and DSS colitis mice. Abbreviations: BPM, beats per minute. DMSO, dimethyl sulfoxide. DSS, dextran sulphate sodium. OFT, open field test. SpO2, blood oxygen saturation. # p<0.05. **, ## p<0.01. *** p<0.001. B, G-H: Two-way ANOVA, Bonferroni test. C-F: One-way ANOVA