FIG. 1.

GLT1 promoter sequence. Putative Gcn4p (GCN4), Gln3p, and Gat1p binding sites (GATA), CGGN₁₅CCG palindrome, and poly(dA-dT) regions are boxed and numbered starting from the most 5′. Two putative TATA boxes (TATA 1 and TATA 2) as well as the two transcription initiation sites, at positions +1 and +52, are indicated. The 714-bp fragment shown includes a 30-bp sequence of the UGA3 coding region. BamHI and SalI sites were added and used to clone this fragment into the 2μm LEU2 lacZ vector YEp363, generating plasmid pLOU1.