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. 1998 Jul;180(14):3533–3540. doi: 10.1128/jb.180.14.3533-3540.1998

FIG. 2.

FIG. 2

Primer extension analysis. (A) Assay of transcription initiation sites (lane PE) of the GLT1 gene, carried out with total RNA obtained from the wild-type strain CLA1. (B) Representative results of primer extension analysis carried out with total RNA obtained from strain CLA1 transformed with plasmid pLOU1, -3, -4, -6, -8, -9, -10, or -11 (lane 1) or with pLOU14, -15, -16, or -17 (lane 2). The sequence ladder was produced with the same deoxyoligonucleotide used for the primer extension reaction (described in Materials and Methods).