FIG. 3.

β-Gal activities of 5′ deletions of the GLT1 promoter. The GLT1 full promoter and 5′ deletions were cloned into the 2μm LEU2 lacZ vector YEp363, generating plasmids pLOU1 to -19. These plasmids were transformed into either the GDH1 wild-type strain CLA1 or the gdh1 mutant strain MAR1. The 5′ region carried in each plasmid is indicated in rows 1 to 19. β-Gal activity was determined in extracts obtained from cells grown on either 0.2% ammonium sulfate or 0.1% glutamate. ND, not detected. Diagrams depict Gcn4p putative binding sites ( ), palindrome (▨^⊓▨), Gln3p putative binding sites (▹, ◃), poly(dA-dT) (▪), putative TATA boxes (*), transcription initiation sites ( , ), and putative URRs.