TABLE 2.
β-Gal and GOGAT specific activities
| Nitrogen source | Sp act (nmol
min−1 mg−1)a
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CLA1-0 (GDH1/YEp363) | CLA1-1 (GDH1/pLOU1) | MAR1-0 (gdh1Δ/YEp363) | MAR1-1 (gdh1Δ/pLOU1) | β-Gal in:
|
|||||||||
| CLA100 (GDH1 gcn4Δ/pLOU1) | CLA101 (GDH1 gln3Δ/pLOU1) | CLA102 (GDH1 gat1Δ/pLOU1) | 27034b (GDH1 UGA43/pSIM1) | 30078c (GDH1 uga43Δ/pSIM1) | |||||||||
| β-Gal | GOGAT | β-Gal | GOGAT | β-Gal | GOGAT | β-Gal | GOGAT | ||||||
| Ammonium | ND | 40 | 1,280 | 41 | ND | 71 | 3,650 | 69 | 600 | 230 | 1,150 | 850 | 630 |
| Proline | ND | 40 | 1,580 | 41 | ND | 53 | 2,360 | 55 | 600 | 290 | 1,250 | 3,850 | 4,250 |
| Glutamate | ND | 22 | 550 | 24 | ND | 24 | 600 | 25 | 200 | 270 | 392 | 910 | 750 |
| Glutamine | ND | 22 | 520 | 21 | ND | 27 | 710 | 29 | 200 | 60 | 362 | 410 | 340 |
| Asparagine | ND | 23 | 450 | 25 | ND | 23 | 450 | 24 | 250 | 100 | 308 | 1,340 | 1,000 |
a
Mean of three independent experiments. Variations were ≤15%. ND, not detected.