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Complementation at ERG3’s endogenous locus with ERG3-3×HA-KanMX rescues erg3Δ and erg3Δerg5Δ phenotypes. (A and B) Fivefold serial dilution spot assays of indicated strains with 0 and 64 µg/mL fluconazole (FLZ) in SC media. Images were captured at 48 hours. (C) Whole cell extracts were isolated and immunoblotted against an anti-HA antibody for the detection of Erg3 in complemented strains. Histone H3 was used as a loading control, and ERG3-3×HA was used as a positive control.
