TABLE 1.
B. subtilis strains used in this study
| Strain | Genotype | Source or derivation and referencea |
|---|---|---|
| LMB1 | trpC2 | E. Ferrari, I168b |
| LMB10 | trpC2 sigD::pLM5 (Cmr) | M. J. Chamberlin, CB100b; 13 |
| LMB24 | trpC2 phe hag::lacZ (Cmr) | M. J. Chamberlin, CB25b; 32 |
| LMB213 | trpC2 flgM::mini-Tn10 (Spr) | Transform [LMB1:HB4229, Spr]; reference 6 and this study |
| LMB214 | trpC2 fla/che PAΔ | Transform [LMB1:pWE4-int, Cmr]c; this study |
| LMB216 | trpC2 fla/che PAΔ flgM::mini-Tn10 (Spr) | Transform [LMB214:LMB213, Spr]; this study |
| LMB219 | trpC2 hag::lacZ (Cmr) | Transform [LMB1:LMB24, Cmr]; this study |
| LMB220 | trpC2 fla/che PAΔ hag::lacZ (Cmr) | Transform [LMB214:LMB24, Cmr]; this study |
| LMB221 | trpC2 flgM::mini-Tn10 (Spr) hag::lacZ (Cmr) | Transform [LMB219:LMB213, Spr]; this study |
| LMB222 | trpC2 flgM::mini-Tn10 (Spr) fla/che PAΔ hag::lacZ (Cmr) | Transform [LMB220:LMB213, Spr]; this study |
a
Transformation of [first (recipient) strain: with chromosomal DNA from second strain, and selecting for resistance indicated] is shown for some strains.
b
Previous designation of strain.
c
This strain was subsequently cured of the chloramphenicol resistance marker.