Extended Data Fig. 9. Inactivating npr-1 regulates pheromone response differently from pathogen exposure.

a, Avoidance of pheromones in wild-type and npr-1 mutant adult hermaphrodites. Pheromones, mixture of ascr#2,3,5 (10 nM each at equilibrium). Positive avoidance index, avoidance.

b - m, Traces of GCaMP signals in ASK (b,d,f) or ADL (h,j,l) neurons of wild-type adult hermaphrodites in response to ascaroside pheromones and quantitation of average GCaMP signals during pheromone stimulation in the previous panel (c,e,g,i,k,m). Phe, pheromone mixture of ascr#2,3,5 at indicated concentrations (b,d,f,h,l). Lines in traces, mean. Shades, s.e.m.. F_baseline, average GCaMP signals in the first 30 seconds.

n, Quantitation of str-44p::gfp signals in AWA in wild-type and npr-1 mutant adult hermaphrodites. Intensity of fluorescence signals is normalized using average intensity of str-44p::gfp in OP50-exposed wild-type worms.

Box plot, median, 1^st and 3^rd quartiles; whiskers, minimum and maximum. Numbers in parenthesis, number of individual assays (a) or individual worms (c,e,g,i,k,m) or individual neurons (n). Dots, Avoidance indexes of individual assays (a) or signals of individual worms (c,e,g,i,k,m) or signals of individual neurons (n). P values are derived from One-way ANOVA with Tukey’s multiple comparisons test (a) or two-tailed unpaired t test (c,g,i,k,m) or two-tailed Mann-Whitney test (e) or Kruskal-Wallis test with Dunn’s multiple comparisons test (n), asterisks indicate significant difference, **** P < 0.0001, *** P < 0.001, ns, not significant. P values are shown in Source data.