Extended Data Fig. 10. Wild-type hermaphrodites exposed to OP50 or PA14 produce male progeny by selfing at a very low frequency, and mating on lawns of different sizes.

a, Cartoon for control experiment.

b, Percentage of male progeny produced by selfing of adult hermaphrodites exposed to OP50 or PA14 for 4 hours. Box plot, median, 1^st and 3^rd quartiles; whiskers, minimum and maximum. Numbers in parenthesis, numbers of control assays (progeny of 10 hermaphrodites were scored for each assay). Dots, male progeny in F1 (%) of individual assays. Two-tailed Mann-Whitney test, ns, not significant. P values are shown in Source data.

c, Cartoon for mating on lawns of different sizes.

d, Frequency of successful mating for adult wild-type hermaphrodites and males on lawns of different sizes. Box plot, median, 1^st and 3^rd quartiles; whiskers, minimum and maximum. Numbers in parenthesis, number of assays (each contains 10 hermaphrodites with their outcome of mating scored individually). Dots, mating frequency of individual assays. P values are derived from One-way ANOVA with Tukey’s multiple comparisons test, asterisks indicate significant difference, **** P < 0.0001, *** P < 0.001. P values are shown in Source data.