Skip to main content
. Author manuscript; available in PMC: 2024 Jan 4.
Published in final edited form as: Nature. 2023 Jan 4;613(7943):324–331. doi: 10.1038/s41586-022-05561-9

Fig. 2. Pathogenic Pseudomonas PA14 induces expression of str-44 in AWA.

Fig. 2.

a, Venn diagram showing numbers of genes differentially expressed in AWA between worms exposed to PA14 or PA14-gacA(−) or P. fluorescens and worms exposed to E. coli OP50 (FDR < 0.1). Numbers in parenthesis, total number of genes in each category.

b, c, Volcano plots showing gene expression difference in AWA between PA14-exposed worms and OP50-exposed worms (b) or between PA14-gacA(−)-exposed worms and OP50-exposed worms (c). Genes with FDR < 0.1 are highlighted in red, otherwise highlighted in black; str-44 is highlighted in green. FC, fold change.

d, Sample images for str-44p::gfp expression in adult hermaphrodites exposed to OP50 or PA14 for 4–6 hours. Dashed lines indicate enlarged views. Arrows indicate neuronal cell bodies. Lines outline worm bodies. Scale bar, 20 μm. A, anterior. D, dorsal (points slightly out of page in lower panel).

e, Quantitation of str-44p::gfp signal in AWA in hermaphrodites exposed to different bacteria. Intensity of signals is normalized using average intensity of str-44p::gfp in OP50-exposed worms measured in parallel. Box plot, median, 1st and 3rd quartiles; whiskers, minimum and maximum. Numbers in parenthesis, number of individual neurons. Dots, signals of individual neurons. P values are derived from Kruskal-Wallis test with Dunn’s multiple comparisons test, asterisks indicate significant difference, **** P < 0.0001, ns, not significant. P values are shown in Source data.