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. Author manuscript; available in PMC: 2024 Jan 4.
Published in final edited form as: Nature. 2023 Jan 4;613(7943):324–331. doi: 10.1038/s41586-022-05561-9

Extended Data Fig. 4. Exposure to pathogenic Serratia bacteria induces pheromone response of AWA.

Extended Data Fig. 4.

a, Cartoon for exposure to different bacteria strains for calcium imaging analysis.

b, c, Traces of GCaMP6 signals in AWA neurons of wild-type adult hermaphrodites in response to ascaroside pheromones after 4–6 hour exposure to different bacteria strains (b) and quantitation of average GCaMP6 signals in AWA during pheromone stimulation (c). Phe, pheromone mixture of ascr#2,3,5 (1 μM each). Lines in traces, mean. Shades, s.e.m.. Fbaseline, average GCaMP6 signals in the first 30 seconds. Box plot, median, 1st and 3rd quartiles; whiskers, minimum and maximum. Numbers in parenthesis, number of individual worms. Dots, average GCaMP6 signals of individual worms during pheromone stimulation. P values are derived from Kruskal-Wallis test with Dunn’s multiple comparisons test, asterisks indicate significant difference, *** P < 0.001, ns, not significant. P values are shown in Source data.

d, e, Cartoon for TRAP-RNAseq (d) and for exposure to different bacteria strains for TRAP-RNAseq analysis (e).