Extended Data Fig. 5. Exposure to PA14 does not induce AWA expression of several previously identified receptors for pheromone sensing.
a - g, Sample images (a - f) and quantitation in AWA neurons (g) for srg-36p::gfp, daf-37p::gfp, srd-1p::gfp, srx-44p::gfp, srbc-64p::gfp, and srbc-66p::gfp expression in adult hermaphrodites exposed to OP50 or PA14 for 4–6 hours (reporters are indicated by the name of the promoters in g). Arrows point to cell bodies of several sensory neurons. Lines outline worm bodies. Scale bar, 20 μm. A, anterior. D, dorsal (arrows point out of the page for OP50 condition in c and for PA14 condition in d). Intensity of fluorescence signals is normalized using average intensity of AWA expression of str-44p::gfp in OP50-exposed worms measured in parallel. Box plot, median, 1st and 3rd quartiles; whiskers, minimum and maximum. Numbers in parenthesis, number of individual neurons. Dots, signals of individual neurons. P values are derived from Two-way ANOVA with Bonferroni’s multiple comparisons test, asterisks indicate significant difference, **** P < 0.0001, ns, not significant. P values are shown in Source data.