Figure 3.

 MAT2A inhibition decreased P. gingivalis-induced inflammatory responses in hGfs.

(a) The viability of hGFs treated with PF9366 was quantified using Cell Counting Kit-8 and hGFs were pretreated with PF9366 (10 μM) and then stimulated with P. gingivalis (MOI = 100) in subsequent experiments. (b) Gene transcription of inflammatory cytokines was assessed by qPCR at 3 h and (c) protein levels were detected by Western blot at 24 h. (d) IL-6, MCP-1, and (e) SAM in the culture supernatants of 24 h were detected by ELISA. Data are presented as the mean ± SD (n = 3). *p < 0.05; **p < 0.01 versus control. #p < 0.05; ##p < 0.01.