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SAM synergistically promotes P. gingivalis-induced inflammation in hGFs.
HGFs were pre-cultured with medium in the presence of SAM (10 μM) for 2 days and then infected with P. gingivalis (MOI = 100) for 3 h for qPCR and 24 h for ELISA. (a) Gene transcription of inflammatory cytokines was assessed by qPCR. (b) IL-6 and MCP-1 in the culture supernatants were detected by ELISA. Data are presented as the mean ± SD (n = 3). *p < 0.05; **p < 0.01.
