. Author manuscript; available in PMC: 2024 Oct 20.

Published in final edited form as: ACS Catal. 2023 Oct 3;13(20):13369–13382. doi: 10.1021/acscatal.3c02404

Table 1.

Characterization of VldE and OtsA chimeras and point mutants.

Enzyme	Sugar or pseudo-sugar donor	Sugar or pseudo-sugar acceptor	Expected product	Product observed
VldE	GDP-valienol	validamine 7-P	validoxylamine A 7´-P	+
VldE-N325S	GDP-valienol	validamine 7-P	validoxylamine A 7´-P	+
VldE-N325S	GDP-glucose	glucose 6-P	trehalose 6-P	–
VldE-Q385M	GDP-valienol	validamine 7-P	validoxylamine A 7´-P	+
VldE-Q385M	GDP-glucose	glucose 6-P	trehalose 6-P	–
VldE-N109H	GDP-valienol	validamine 7-P	validoxylamine A 7´-P	+
VldE-N109H	GDP-glucose	glucose 6-P	trehalose 6-P	–
VldE-D158N	GDP-valienol	validamine 7-P	validoxylamine A 7´-P	–
	GDP-glucose	validamine 7-P	pseudo-aminotrehalose 7´-P	+
	GDP-glucose	glucose 6-P	trehalose 6-P	–
VldE-H182A	GDP-valienol	validamine 7-P	validoxylamine A 7´-P	–
	GDP-glucose	validamine 7-P	pseudo-aminotrehalose 7´-P	+
	GDP-glucose	glucose 6-P	trehalose 6-P	–
VldE-D383N	GDP-valienol	validamine 7-P	validoxylamine A 7´-P	+
VldE-D383N	GDP-glucose	glucose 6-P	trehalose 6-P	–
VldE-K11N/L178G	GDP-valienol	validamine 7-P	validoxylamine A 7´-P	+
VldE-K11N/L178G	GDP-glucose	glucose 6-P	trehalose 6-P	–
VldE-L8V/N109H/T135N	GDP-valienol	validamine 7-P	validoxylamine A 7´-P	+
	GDP-glucose	glucose 6-P	trehalose 6-P	–
	GDP-valienol	glucosamine 6-P	val-GlcN 6´-P	–
	GDP-glucose	glucosamine 6-P	2-amino-trehalose 6-P	–
Chimera-1-N109H	GDP-valienol	validamine 7-P	validoxylamine A 7´-P	+
Chimera-1-N109H	GDP-glucose	glucose 6-P	trehalose 6-P	–
Chimera-1-D158N	GDP-glucose	validamine 7-P	pseudo-aminotrehalose 7´-P	+
Chimera-1-H182A	GDP-glucose	validamine 7-P	pseudo-aminotrehalose 7´-P	+
Chimera-1-K11N/L178G	GDP-valienol	validamine 7-P	validoxylamine A 7´-P	+
Chimera-1-K11N/L178G	GDP-glucose	glucose 6-P	trehalose 6-P	–
Chimera-1-L8V/N109H/T135N	GDP-valienol	validamine 7-P	validoxylamine A 7´-P	+
	GDP-glucose	glucose 6-P	trehalose 6-P	–
	GDP-valienol	glucosamine 6-P	val-GlcN 6´-P	–
	GDP-glucose	glucosamine 6-P	2-amino-trehalose 6-P	–
Chimera-3	GDP-valienol	validamine 7-P	validoxylamine A 7´-P	–
	GDP-glucose	glucose 6-P	trehalose 6-P	+
	GDP-glucose	validol 7-P	pseudo-trehalose 7´-P	+
OtsA^*	GDP-valienol	validamine 7-P	validoxylamine A 7´-P	–
OtsA^*	GDP-glucose	glucose 6-P	trehalose 6-P	+
OtsA-H154A	GDP-valienol	validamine 7-P	validoxylamine A 7´-P	–
OtsA-H154A	GDP-glucose	glucose 6-P	trehalose 6-P	+

see Abuelizz and Mahmud²⁹