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. 2023 Dec 20;4(6):e463. doi: 10.1002/mco2.463

FIGURE 2.

FIGURE 2

USP20 knockdown confers ferroptosis of HCC cells. (A) knockout efficiency of USP20. (B and C) The relative cell viability of Huh‐7 and Hep3B cells treated with the indicated dosage of erastin for 24 h. (Each group contained three replicates.) (D and E) Crystal violet staining of HCC cells treated with erastin. (F and G) CCK8 assay showing the response of HCC cell lines to erastin (20 μM) ± ferrostatin (1 μM) for 24 h. (H–M) Lipid ROS (H and I), GSH levels (J and K), and ferrous iron levels (L and M) were measured in HCC cells. Results shown are representative of three independent experiments. Data are represented as mean ± SD of biological triplicates.*p value < 0.05**, p value < 0.01, ***p value < 0.001.