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. 2023 Dec 8;21(12):e3002431. doi: 10.1371/journal.pbio.3002431

Fig 3. AcrIIA1 induces degradation of SauCas9 but not Cas12a.

Fig 3

(A) Editing efficiencies for SauCas9 targeting Chrm2. AcrIIA1 only modestly inhibits SauCas9 at a 1:1 plasmid ratio (AcrIIA1 (1x), P = 0.01). Bars represent the mean of biological replicates (dots). Underlying data can be found in S1 Data. (B) Western blot of SauCas9 co-expressed with various Acr proteins. Co-expression of AcrIIA1 induces degradation of SauCas9 similarly to SpyCas9. AcrIIA4 and AcrVA1 do not affect SauCas9 protein concentrations. (C) Western blot of AsCas12a co-expressed with various Acr proteins. AsCas12a protein concentration is not affected by either AcrIIA1 or AcrIIA4.