Figure 3: Severe downregulation of the FFAR by maternal EtOH use.
OL-Es from the plasmas were measured for FFAR protein by ELISA (A). The same plasma from pregnant women who drank EtOH or did not use EtOH were measured (in triplicates) for FFAR by ELISA (B) and flow cytometry (C). Downregulation was greatest in the cases with EtOH exposure (graphs show means from triplicate assays +/− SD). Downregulation was statistically significant (p<0.05). For absolute quantitation of MBP by ELISA, values are shown in ng/mL (normalized to CD81).