Skip to main content
. Author manuscript; available in PMC: 2024 Jun 1.
Published in final edited form as: Nat Chem Biol. 2023 Jun 22;19(12):1458–1468. doi: 10.1038/s41589-023-01353-y

Extended Data Fig.4. FAK-SPARK expression does not perturb dynamics of focal adhesion assembly and disassembly.

Extended Data Fig.4

A & B, Representative images of HeLa cells expressing mApple-paxillin + FAK-SPARK and mApple-paxillin + GFP, respectively. C & D, Tracking of FAs using Focal Adhesion Analysis Server {Steenkiste:2021iv, Berginski:2013dj} in A & B. E, A typical trace of assembly and disassembly rate calculation. F & G, Assembly rate and disassembly rate in HeLa cells expressing FAK-SPARK or GFP. Data represent mean ± SE (n ~300 focal adhesions) n.s., not significant. Scale= 10 μm.