Skip to main content
. 2023 Dec 20;9(51):eadj1397. doi: 10.1126/sciadv.adj1397

Fig. 1. PAD4 is activated by apoptotic and necroptotic stimuli and controls cytoplast formation.

Fig. 1.

(A and B) Protein expression levels of H3Cit, pMLKL, (cl.) caspase 3, and Erk1/2 in freshly isolated WT and Padi4−/− BM neutrophils stimulated with apoptotic and necroptotic stimuli. (C) Flow cytometry evaluation of cell size (FSC-A) and granularity (SSC-A), propidium iodide (PI), annexin V (AnnV), CellTracker Green (CTG), and Hoechst in WT and Padi4−/− neutrophils stimulated as indicated. (D) Statistical analysis of the proportion of each cell cluster identified by dimension reduction (UMAP) and automated clustering (FLOWSOM) from (C). Means ± SEM, n = 3 in each stimuli group, *P < 0.05, **P < 0.005, ***P < 0.0005, ****P < 0.0001, WT versus Padi4−/−, unpaired t test. n.s., not significant.