Figure 3.

Misprocessing results in the preservation of splicing signals, which promote nuclear retention. Properly processed mRnas are compared to misprocessed mRnas that generate IPA transcripts. Note that the IPA transcript contains both an intact 5’SS due to the failure of splicing, and m6A modifications, due to the lack of deposited EJCs, which normally inhibits m6A modifications around the splice site. This could be due to the EJC sterically preventing the methylatransferase from accessing the mRNA (as depicted in the figure) or by the recruitment of demethylases such as ALKBH5.