FIG. 2.

Nucleoid segregation in metK filaments. A culture of strain MEW402 was grown for 19 h with 25 μg of leucine per ml and prepared for fluorescence microscopy as described in Materials and Methods; control cells grown with 50 μg/ml are shown in the inset. It can be seen that the nucleoids are segregated uniformly along the length of the filament. Most nucleoid regions are in the form of a dumbbell, which presumably represents two not fully separated chromosomes. Analysis of the relative amount of DNA per individual nucleoid region by integrated density measurement shows that this filament contains 16 nucleoid equivalents. Bar, 5 μm.