TABLE 2.
SAM synthetase activity of metK and lrp mutants
| Mediuma | SAM synthetase activity (nmol/min/mg of protein) in:
|
|||
|---|---|---|---|---|
| WTb | lrp | metK84 | lrp metK84 | |
| −Leucine | 2.3 | 4.6 | 0.04 | 0.28 |
| +Leucine | 2.9 | 6.3 | 0.26 | 0.29 |
a
Cultures were grown in glucose-minimal medium with (+) and without (−) 100 μg of leucine per ml, and SAM synthetase was assayed. The cultures assayed were also examined with a microscope; all cells were short rods, except for cells of the metK84 mutant grown without leucine, which consisted mainly of filaments. The cultures used were MEW1 (wild type [WT]), MEW45 (lrp::kan), MEW402 (metK84), and MEW403 (lrp::kan metK84).